A

Abomination – Tales to Astonish #90 (1967)

Absorbing Man – Journey Into Mystery #114 (1965)

Amazo – Brave and the Bold (JLA) #30 (1960)

Apocalypse – X-Factor #5 (1986)

.

B

Bane – Batman: Vengeance of Bane #1 (1993)

Baron Zemo – Avengers #6 (1964)

Beyonder – Secret Wars #1 (1984)

Bizarro – Superboy #68 (1958)

Black Adam (Fawcett/DC) – Marvel Family #1 (1945)

Black Manta – Aquaman #35 (1967)

Black Mask – Batman #389 (1985)

Blacksmith – Flash: Iron Heights #1 (2001)

Blastarr – Fantastic Four #62 (1967)

Blob – X-men #3 (1964)

Bolivar Trask – X-Men #14 (1965)

Brainiac – Action Comics #242 (1958)

Brotherhood of Evil Mutants – X-men #4 (1964)

Bullseye – Daredevil #131 (1976)

.

C

Captain Boomerang – Flash #117 (1960)

Captain Cold – Showcase #8 (1957)

Carnage – Amazing Spider-man #361 (1st Full App.) (1993)

Catwoman –  1st Full App. as Catwoman in Costume Batman #3 (1940)

Chameleon – Amazing Spider-Man #1 (1963)

Cheetah – Wonder Woman #6 (1943)

Clayface – Detective Comics #40 (194)

Constrictor – Incredible Hulk #212 (1977)

Crime Syndicate of America – Justice League of America #29 (1964)

Crossbones – Captain America #359 (1989)

Crossfire – Marvel Two-In-One #52 (1979)

Cyber – Marvel Comics Presents #85 (cameo)/ #86 (full – 1991)

.

D

Dark Phoenix – Uncanny X-Men #139 (1980)

Darkseid – Superman’s Pal Jimmy Olsen #134 (1970)

Deadpool – New Mutants #98 (1991)

Deadshot – Batman #50 (1950)

Deathlok – Astonishing Tales #25 (1974)

Deathstroke – New Teen Titans #2 (1980)

Doctor Doom – Fantastic Four #5 (1962)

Doc Octopus – Amazing Spiderman #3 (1963)

Doomsday – 1st Full App. Superman: The Man of Steel #18 (1993)

Doctor Light – Justice League of America #12 (1962)

Dormammu – Strange Tales #126 (1964)

.

E

Egghead – Tales to Astonish #38 (1962)

Electro – Amazing Spiderman #9 (1964)

Enchantress – Journey Into Mystery #103 (1964)

Equus – Superman, Vol 2 #206 (2004)

Evil Ernie – Evil Ernie #1 (1991)

Executioner (Skurge) – Journey Into Mystery #103 (1964)

.

F

Fabian Cortez – X-MenVol. 2 #1 (1991)

Famine (Horseman) – X-Factor #12 (1987)

Felix Faust – Justice League of America #10 (1962)

Fin Fang Foom – Strange Tales #89 (1961)

Firebug – Batman #318 (1979)

Frightful Four – Fantastic Four #36 (1966)

G

Galactus – Cameo Fantastic Four #48 1st Full App. Fantastic Four #49 (1966)

Gladiator (Melvin Potts) – Daredevil #18 (1966)

Graviton – Avenger #158

Green Goblin – (Norman Osborn) Amazing Spiderman #14 (1964)

.

H

Harley Quinn –  The Batman Adventures #12 (1994)

High Evolutionary – Thor #134 (1966)

Hobgoblin – Amazing Spiderman #238 (1983)

Hood (Parker Robbins) – The Hood #1 (2002)

Hugo Strange – Detective Comics #36 (1940)

.

J

Jackal – Amazing Spider-Man #129 (1974)

Jigsaw (Punisher)- Amazing Spiderman #162 (1976)

Joker – Batman #1 (1940)

Juggernaut – X-men #12 (1965)

.

K

KGBeast – Batman #417 (1988)

Killer Frost –  (Crystal Frost) Firestorm #3 (June 1978); (Louise Lincoln) Firestorm (vol. 2) # 21 (March 1984); (Caitlin Snow) Fury of Firestorm: The Nuclear Man #19 (June 2013)

Kingpin – Amazing Spiderman #50 (1967)

Kraven the Hunter – Amazing Spiderman #15 (1964)

.

L

Lady Death – Evil Ernie #1 (1991)

Lady Deathstrike – Alpha Flight #33 (1986)

Leader – Tales to Astonish #62 (1965)

Legion (David Haller) – New Mutants #25 (1985)

Lex Luthor – Action Comics #23 (1940)

Livewire – Superman Adventures #5 (1997)

Living Laser – Avengers #34 (1966)

Lizard – Amazing Spiderman #6 (1963)

Lobo – Omega Men #3 (1983)

Loki – Journey Into Mystery – #85 (1962)

.

M

Mad Hatter – Batman #49 (1948)

Mad Jim Jaspers – Marvel Superheroes #377 (1981)

Mad Thinker – Fantastic Four #15 (1963)

Magenta – New Teen Titans #17 (1982)

Magneto – X-men #1 (1963)

Magus (Adam Warlock) – Strange Tales #178 (1975)

Magus (X-Men) – New Mutants #18 (1984)

Major Disaster – Green Lantern Vol. 2 #43 (1966)

Man-Bat – Detective Comics #400 (1970)

Mandarin – Tale of Suspense #50 (1964)

Marauders – Uncanny X-men #210 (1986)

Marquis of Death – 1985 #2 (2008) as Clyde Wyndcham; Fantastic Four #562 (2009) as Marquis

Marvel Zombies – Ultimate Fantastic Four #21 (2005)

Mastermind – X-Men #4 (1964)

Master Mold – X-Men #15 (1965)

Masters of Evil – Avengers #6 (1964)

Maximus – Fantastic Four #47 (1966)

Mephisto – Silver Surfer Vol. 1 #3 (1968)

Metallo – Action Comics #252 (1959)

Mimic – X-men #19 (1966)

Mirror Master – The Flash #105 (1959)

Mister Hyde – Journey Into Mystery #99 (1963)

Mister Sinister – X-men #221 (1989)

Modok – Tales of Suspense #94 (1967)

Mojo –  Longshot #3 (1985)

Molecule Man – Fantastic Four #20 (1963)

Mole Man – Fantastic Four #1 (1961)

Molten Man – Amazing Spiderman #28 (1965)

Mongul – DC Comics Presents #27 (1980)

Morgan le Fey – Black Knight #1 (1955)

Morlun – Amazing Spider-Man #30 vol. 2 (2001)

Mr. Freeze – Batman #121 (1959)

Mxyztplk – Superman #30 (1944)

Mysterio – Amazing Spiderman #13 (1964)

Mystique – Ms. Marvel #16 (1978)

.

N

Nightmare – Strange Tales #110 (1963)

Nitro – Captain Marvel #34 (1974)

Norman Osborn – Amazing Spider-Man #14 (1964)

Nuke – Daredevil #232 (1985)

.

O

Ocean Master – Aquaman #29 (1966)

Omega Men – Green Lantern #141 (1981)

Omega Red – X-men #4, vol. 2 (1991)

Onslaught – X-Man #15 (1996)

Owl – Daredevil #3, Vol 1 (1964)

.

P

Penguin – Detective Comics #58 (1941)

Phalanx – Uncanny X-men #311 (1994)

Poison Ivy – Batman #181 (1966)

r

Purple Man – Daredevil #4 (1963)

.

R

Ra’s al Ghul – Batman #232 (1971)

Reverse-Flash – The Flash #139 (1963)

Red Skull – Captain America Comics #7 (1941)

Rhino – Amazing Spiderman #41 (1966)

Riddler – Detective Comics #140 (1948) Silver Age App. Batman #171 (1965)

.

S

Sabretooth – Iron Fist #14 (1977)

Saint of Killers – Preacher #2 (1995)

Sandman – Amazing Spiderman #4 (1963)

Scarecrow – World’s Finest Comics #3 (1941)

Scorpion – Amazing Spiderman #20 (1964)

Sentinels – X-men #14 (1965)

Sinestro – Green Lantern #7 (1961)

Speed Demon – Amazing Spider-Man #222 (1981)

Spiral – Longshot #1 (1985)

Stilt-Man – Daredevil #8 (1965)

.

T

Talia (Ra’s al Ghul’s Daughter) – Detective Comics #411 (1971)

Taskmaster – Avengers #195 (Cameo) #196 (1st Full App.) (1980)

Thanos – Iron Man #55 (1973)

Thinker – All Flash #12 (1943)

Thunderbolts – Incredible Hulk #449 (1997)

Toad – X-men #4 (1964)

Two Face – Detective Comics #66 (1942) Silver Age App. Batman #234 (1971)

.

U

Ultron – Avengers #54 cameo, Avengers #55 Full (1968)

Unus the Untouchable – (Uncanny) X-Men #8 (1963)

V

Venom – (cameos) Amazing Spiderman #298-299 – #300 (1st Full Appearance) (1988)

Vixen – Action Comics #521 (1981)

Vulture – Amazing Spiderman #2 (1963)

Vulcan (Gabriel Summers) – X-Men: Deadly Genesis #1

.

W

Wendigo – Incredible Hulk #162 (1973)

Whiplash (Iron Man) – Tales of Suspense #97 (1968)

White Queen (Emma Frost) – X-men #129 (1980)

.

X

Xemnu – Journey Into Mystery #62 (1960)

Y

Yancy Street Gang – Fantastic Four #14 (1963)

Z

Zaran – Master of Kung-Fu #77 (1979)

Zoom – Flash Vol. 3 #197 (2003)

Zsasz (Victor) – Batman: Shadow of the Bat #1 (1992)